Focusing Transgene Expression in Drosophila by Coupling Gal4 With a Novel Split-LexA Expression System [Methods, technology, and resources]

Focusing Transgene Expression in Drosophila by Coupling Gal4 With a Novel Split-LexA Expression System [Methods, technology, and resources]: "

Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.

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