Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq: "

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

Nature Methods 8, 565 (2011).
doi:10.1038/nmeth.1626

Authors: Pattabhiraman Shankaranarayanan, Marco-Antonio Mendoza-Parra, Mannu Walia, Li Wang, Ning Li, Luisa M Trindade & Hinrich Gronemeyer

Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells.

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